Bejing Chuiyangliu Hospital 1 article published in JoVE Medicine Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice Kui Wu1, Lin-Ling Li2, Yu-Kun Li1, Xiao-Dong Peng1, Meng-Xia Zhang1, Ke-Sen Liu1, Xue-Si Wang1, Jia-Xue Yang1, Song-Nan Wen1, Yan-Fei Ruan1, Nian Liu1, Rong Bai1 1Department of Cardiology, Bejing Anzhen Hospital, Capital Medical University, 2Department of Cardiology, Bejing Chuiyangliu Hospital This protocol describes modifications of the Langendorff method including the depth of aorta cannulation for simultaneous Isolation of atrial and ventricular myocytes from adult mice.