National Institute for Biotechnology in the Negev (NIBN) 1 article published in JoVE Biochemistry A Colorimetric Method for Measuring Iron Content in Plants Jonas C. Gitz1,2, Noy Sadot1, Michele Zaccai1, Raz Zarivach1,2 1Department of Life Sciences, Ben-Gurion University of the Negev, 2National Institute for Biotechnology in the Negev (NIBN) We present a simple and reliable protocol for measuring iron content in plant tissues using the colorimetric Prussian Blue method.