German Sports University Cologne 1 article published in JoVE Developmental Biology Assessment of Endothelial Cell Migration After Exposure to Toxic Chemicals Dirk Steinritz1,2, Annette Schmidt1,3, Frank Balszuweit1, Horst Thiermann1, Marwa Ibrahim3, Birgit Bölck3, Wilhelm Bloch3 1Bundeswehr Institute of Pharmacology and Toxicology, 2Walther Straub Institute of Pharmacology and Toxicology, Ludwig-Maximilians-Universität München, 3Department of Molecular and Cellular Sports Medicine, German Sports University Cologne Investigation of early endothelial cell (EEC) migration is important to understand the pathophysiology of certain illnesses and to potentially identify novel strategies for therapeutic intervention. The following protocol describes techniques to assess cell migration that have been adapted for the investigation of EEC.