Maria Sklodowka-Curie Institute - Oncology Center 1 article published in JoVE Developmental Biology Neurogenesis Using P19 Embryonal Carcinoma Cells Paweł Leszczyński1, Magdalena Śmiech1, Aamir S. Teeli1, Aleksandra Zołocińska2, Anna Słysz2, Zygmunt Pojda2, Mariusz Pierzchała3, Hiroaki Taniguchi1 1Department of Experimental Embryology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, 2Department of Regenerative Medicine, Maria Skłodowska-Curie Institute - Oncology Center, 3Department of Genomics and Biodiversities, Institute of Genetics and Animal Breeding, Polish Academy of Sciences The P19 mouse embryonic carcinoma cell line (P19 cell line) is widely used for studying the molecular mechanism of neurogenesis with great simplification compared to in vivo analysis. Here, we present a protocol for retinoic acid-induced neurogenesis in the P19 cell line.