CBIOS - Universidade Lusofona‘s Research Center for Biosciences and Health Technologies 1 article published in JoVE Bioengineering Multispectral Optoacoustic Tomography for Functional Imaging in Vascular Research Tiago Granja1, Sérgio Faloni de Andrade1, Luís Monteiro Rodrigues1 1CBIOS - Universidade Lusófona‘s Research Center for Biosciences and Health Technologies The present protocol describes the acquisition of multispectral optoacoustic images of in vivo human skin vasculature. These include the quantification of hemoglobin and melanin, regarded as chromophores of interest for functional analysis.