Idaho College of Osteopathic Medicine 2 articles published in JoVE Biology Effective Oral RNA Interference (RNAi) Administration to Adult Anopheles gambiae Mosquitoes Mabel Taracena1,2, Catherine Hunt1, Pamela Pennington3, Deborah Andrew4,5, Marcelo Jacobs-Lorena5,6, Ellen Dotson1, Michael Wells5,7,8 1Division of Parasitic Diseases and Malaria, Entomology Branch, Centers for Disease Control and Prevention, 2Department of Entomology, Cornell University, 3Centro de Estudios en Biotecnologia, Universidad del Valle de Guatemala, 4Department of Cell Biology, Johns Hopkins School of Medicine, 5Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, 6Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health and Malaria Research Institute, 7Department of Cell Biology, Johns Hopkins School of Medicine, 8Biomedical Sciences Department, Idaho College of Osteopathic Medicine The oral administration of dsRNA produced by bacteria, a delivery method for RNA interference (RNAi) that is routinely used in Caenorhabditis elegans, was successfully applied here to adult mosquitoes. Our method allows for robust reverse genetics studies and transmission-blocking vector studies without the use of injection. Biology Dissection and Immunostaining of Larval Salivary Glands from Anopheles gambiae Mosquitoes Michelle Z. Chiu1,2, Steven Lannon1, Marisol Luchetti1, Michael B. Wells3, Deborah J. Andrew1 1Department of Cell Biology, The Johns Hopkins University School of Medicine, 2Tufts School of Medicine, 3Department of Biomedical Sciences, Idaho College of Osteopathic Medicine The adult mosquito salivary gland (SG) is required for the transmission of all mosquito-borne pathogens to their human hosts, including viruses and parasites. This video demonstrates efficient isolation of the SGs from the larval (L4) stage Anopheles gambiae mosquitoes and preparation of the L4 SGs for further analysis.
