EyeNexo, LLC 1 article published in JoVE Medicine Using an Automated Hirschberg Test App to Evaluate Ocular Alignment Gang Luo1, Shrinivas Pundlik1, Matteo Tomasi2, Kevin Houston1 1Schepens Eye Research Institute, Mass Eye & Ear, Harvard Medical School, 2EyeNexo, LLC We present a protocol of using a smartphone app to perform Hirschberg test for measuring manifest and intermittent ocular misalignment (strabismus) under near and far fixation conditions.