University of Maryland, Baltimore View Institution's Website 9 articles published in JoVE Biochemistry Platform Incubator with Movable XY Stage: A New Platform for Implementing In-Cell Fast Photochemical Oxidation of Proteins Danté Johnson1, Benjamin Punshon-Smith2, Jessica A. Espino1, Anne Gershenson3, Lisa M. Jones1 1Department of Pharmaceutical Sciences, University of Maryland Baltimore, 2Technology Research Center, University of Maryland Baltimore County, 3Department of Biochemistry and Molecular Biology, University of Massachusetts A new static platform is used to characterize protein structure and interaction sites in the native cell environment utilizing a protein footprinting technique called in-cell fast photochemical oxidation of proteins (IC-FPOP). Biochemistry Characterizing Cellular Proteins with In-cell Fast Photochemical Oxidation of Proteins Emily E. Chea1, Aimee Rinas2, Jessica A. Espino1, Lisa M. Jones1 1Department of Pharmaceutical Sciences, University of Maryland Baltimore, 2AIT Bioscience Here, we characterize protein structure and interaction sites in living cells using a protein footprinting technique termed in-cell fast photochemical oxidation of proteins (IC-FPOP). Medicine Esophageal Heat Transfer for Patient Temperature Control and Targeted Temperature Management Melissa I. Naiman1,2, Maria Gray2, Joseph Haymore3, Ahmed F. Hegazy4, Andrej Markota5, Neeraj Badjatia6, Erik B. Kulstad2,7 1Center for Advanced Design, Research, and Exploration, University of Illinois at Chicago, 2Attune Medical, 3University of Maryland School of Nursing, 4University of Western Ontario, 5University Medical Centre Maribor, 6University of Maryland, 7Department of Emergency Medicine, University of Texas, Southwestern Medical Center This study presents a novel method to provide efficient patient temperature control for cooling or warming patients. A single use, triple lumen device is placed into the esophagus, analogous to a standard orogastric tube, and connects to existing heat exchange units to perform automatic patient temperature management. Biology A Quantitative Assay to Study Protein:DNA Interactions, Discover Transcriptional Regulators of Gene Expression, and Identify Novel Anti-tumor Agents Karen F. Underwood1, Maria T. Mochin1, Jessica L. Brusgard2, Moran Choe3, Avi Gnatt4, Antonino Passaniti3,5 1Greenebaum Cancer Center, University of Maryland School of Medicine, 2Program in Molecular Medicine, University of Maryland School of Medicine, 3Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine, 4Department of Pharmacology & Experimental Therapeutics, University of Maryland School of Medicine, 5Department of Pathology and Biochemistry & Molecular Biology, University of Maryland School of Medicine We developed a quantitative DNA-binding, ELISA-based assay to measure transcription factor interactions with DNA. High specificity for the RUNX2 protein was achieved with a consensus DNA-recognition oligonucleotide and specific monoclonal antibody. Colorimetric detection with an enzyme-coupled antibody substrate reaction was monitored in real time. Neuroscience The Mouse Forced Swim Test Adem Can1, David T. Dao2, Michal Arad1, Chantelle E. Terrillion3, Sean C. Piantadosi1, Todd D. Gould1,3,4 1Department of Psychiatry, University of Maryland School of Medicine, 2Tulane University School of Medicine, 3Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, 4The Program in Neuroscience, University of Maryland The forced swim test is validated as an experimental approach to assess potential antidepressant efficacy in rodents. Experimental animals are placed in a tank of water and escape-related mobility behavior is quantified. The common procedures for the mouse version of this test are described. Neuroscience The Tail Suspension Test Adem Can*1, David T. Dao*1,2, Chantelle E. Terrillion3, Sean C. Piantadosi1, Shambhu Bhat1, Todd D. Gould1,3,4 1Department of Psychiatry, University of Maryland School of Medicine, 2Tulane University School of Medicine, 3The Program in Neuroscience, University of Maryland, 4Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine The tail-suspension test is validated as an experimental procedure to assess antidepressant efficacy of drug treatments in mice. Mice are suspended by their tails for six minutes and escape-related behaviors are assessed. We describe procedures used in conducting the tail suspension test. Medicine An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery Richard M. Lovering1,2, Joseph A. Roche1, Mariah H. Goodall2, Brett B. Clark2, Alan McMillan3 1Department of Physiology, University of Maryland School of Medicine, 2Department of Orthopaedics, University of Maryland School of Medicine, 3Department of Diagnostic Radiology, University of Maryland School of Medicine An in vivo animal model of injury is described. The method takes advantage of the subcutaneous position of the fibular nerve. Velocity, timing of muscle activation, and arc of motion are all pre-determined and synchronized using commercial software. Post injury changes are monitored in vivo using MR imaging/spectroscopy. Medicine Manual Muscle Testing: A Method of Measuring Extremity Muscle Strength Applied to Critically Ill Patients Nancy Ciesla1, Victor Dinglas1, Eddy Fan1,2, Michelle Kho2,3, Jill Kuramoto4, Dale Needham1,2,3 1Outcomes After Critical Illness and Surgery (OACIS) Group, Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, 2Critical Care Physical Medicine and Rehabilitation Program, Johns Hopkins Hospital, 3Department of Physical Medicine and Rehabilitation, Johns Hopkins University, 4Department of Rehabilitation Services, University of Maryland Medical System Survivors of acute respiratory distress syndrome (ARDS) and critical illness frequently develop long-lasting muscle weakness. Manual muscle testing (MMT) is a standardized clinical examination commonly used to measure strength of peripheral skeletal muscle groups. This video demonstrates MMT using the 6-point Medical Research Council scale. Bioengineering Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points Conrad W. Liang*1, Michael Mohammadi*1, M. Daniel Santos1, Cha-Min Tang1 1Department of Neurology, Baltimore VA Medical Center, University of Maryland School of Medicine Digital micromirror devices (DMD) can generate complex patterns in time and space with which to control neuronal excitability. Issues relevant to the design, construction, and operation of DMD systems are discussed. Such a system enabled the demonstration of non-linear integration across distal dendritic branch points.