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Homemade plaatsgerichte mutagenese van Whole Plasmids

Article DOI: 10.3791/1135-v • 07:12 min • May 11th, 2009
May 11th, 2009 •
1, 2
1Department of Biology, Johannes Gutenberg-University Mainz, Germany, 2Proteomics division, AlPlanta, Neustadt an der Weinstrasse, Germany

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Plaatsgerichte mutagenese van hele plasmiden is een eenvoudige manier om iets verschillende varianten van een origineel plasmide te creëren. Hier laten we zien op een eenvoudige en kosteneffectieve manier om de base substituties te introduceren in een plasmide met behulp van standaard reagentia.

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Basic protocollen plaatsgerichte mutagenese mutagenese mutatie Plasmide thermocycling PCR Pfu-polymerase Dpn1 kostenbesparing
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