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Hausgemachte Mutagenese von Whole Plasmide

Article DOI: 10.3791/1135-v • 07:12 min • May 11th, 2009
May 11th, 2009 •
1, 2
1Department of Biology, Johannes Gutenberg-University Mainz, Germany, 2Proteomics division, AlPlanta, Neustadt an der Weinstrasse, Germany

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Seite Mutagenese ganzer Plasmide ist ein einfacher Weg, um etwas anders Variationen eines Original-Plasmid zu erzeugen. Hier zeigen wir eine einfache und kostengünstige Möglichkeit, Basensubstitutionen in ein Plasmid mit Standard-Reagenzien einzuführen.

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Grundlegende Protokolle Mutagenese Mutagenese Mutation Plasmide Thermocycling PCR Pfu-Polymerase Dpn1 Kostenersparnis
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