Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Chemistry / Produktion af disulfid-stabiliserede…
JoVE Journal
Chemistry

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE Journal Chemistry
Produktion af disulfid-stabiliserede transmembrane peptidkomplekser til strukturelle studier
NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements
Journal (Chemistry)
NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements
Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy
Journal (Chemistry)
Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy
 
Click here for the English version

Produktion af disulfid-stabiliserede transmembrane peptidkomplekser til strukturelle studier

Article DOI: 10.3791/50141-v • 12:05 min • March 6th, 2013
March 6th, 2013 •
1,2, 1, 1,2, 1,2, 1,2, 1,2
1Structural Biology Division, The Walter and Eliza Hall Institute of Medical Research, 2The University of Melbourne

챕터

요약
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Biofysiske og biokemiske studier af samspillet mellem membran-embedded proteindomæner står over for mange tekniske udfordringer, hvoraf den første er at opnå passende undersøgelse materiale. I denne artikel beskrives en protokol til fremstilling og oprensning af disulfid-stabiliserede transmembrane peptidkomplekser, der er egnede til strukturel analyse ved opløsning kernemagnetisk resonans (NMR) og andre analytiske anvendelser.

Tags

Biochemistry Structural Biology Chemistry Chemical Engineering Biophysics Genetics Molecular Biology membranproteiner Proteins Molecular Structure transmembrant domæne peptidkemi membranprotein struktur immune receptorer omvendt fase-HPLC HPLC peptider lipider protein kloning TFA Elution CNBr Digestion NMR ekspression cellekultur
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter