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Bioengineering

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JoVE Journal Bioengineering
Evaluering af Polymeric Gene Delivery nanopartikler ved Nanopartikel Tracking Analyse og Højkapacitetsforskning Flowcytometri
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Evaluering af Polymeric Gene Delivery nanopartikler ved Nanopartikel Tracking Analyse og Højkapacitetsforskning Flowcytometri

Article DOI: 10.3791/50176-v • 08:51 min • March 1st, 2013
March 1st, 2013 •
1,2, 1,2, 1,2,3,4
1Biomedical Engineering Department, Johns Hopkins University School of Medicine, 2Translational Tissue Engineering Center, Johns Hopkins University School of Medicine, 3Wilmer Eye Institute, Johns Hopkins University School of Medicine, 4Institute for Nanobiotechnology, Johns Hopkins University School of Medicine

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En protokol til nanopartikler sporing analyse (NTA) og højkapacitetsidentifikation flowcytometri for at evaluere polymere genafgivelsessystemer nanopartikler beskrives. NTA anvendes til at karakterisere nanopartikel partikelstørrelsesfordeling og plasmidet pr partikelfordeling. High-throughput flowcytometri muliggør kvantitativ transfektion Effektevalueringen for et bibliotek af gen-leveringsvehikler biomaterialer.

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Biomedical Engineering Bioengineering Tissue Engineering Cellular Biology medicin genetik biokompatible materialer Biopolymers Drug Delivery Systems Nanoteknologi bioteknologi (generelt) Therapeutics Nanopartikel poly (beta-amino ester) high-throughput transfektion nanopartikel sporing analyse biomaterialet genlevering flowcytometri
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