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Evaluering av polymere Gene levering Nanopartikler av nanopartikler Tracking Analysis og High-throughput flowcytometri

Article DOI: 10.3791/50176-v • 08:51 min • March 1st, 2013
March 1st, 2013 •
1,2, 1,2, 1,2,3,4
1Biomedical Engineering Department, Johns Hopkins University School of Medicine, 2Translational Tissue Engineering Center, Johns Hopkins University School of Medicine, 3Wilmer Eye Institute, Johns Hopkins University School of Medicine, 4Institute for Nanobiotechnology, Johns Hopkins University School of Medicine

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En protokoll for nanopartikkel sporing analyse (NTA) og high-throughput flowcytometri å vurdere polymere genet levering nanopartikler er beskrevet. NTA utnyttes for å karakterisere nanopartikkel partikkelstørrelsesfordeling og plasmidet per partikkel fordeling. High-throughput flowcytometri gjør kvantitative transfeksjon effektevalueringen for et bibliotek av genet levering biomaterialer.

Tags

Biomedical Engineering bioteknologi Tissue Engineering cellebiologi medisin genetikk biokompatible materialer Biopolymere Drug Delivery Systems nanoteknologi bioteknologi (generelt) Therapeutics nanopartikler poly (beta-aminoester) høy gjennomstrømning transfeksjon nanopartikler sporing analyse biomateriale genet levering flowcytometri
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