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JoVE Journal Biology
Een Kwantitatieve Analyse naar Protein Studie
Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy
Journal (Biology)
Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy
DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses
Journal (Biology)
DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses
 
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Een Kwantitatieve Analyse naar Protein Studie: DNA interacties, Discover transcriptieregulatoren van genexpressie, en het identificeren van nieuwe anti-tumor Agents

Article DOI: 10.3791/50512-v • 06:43 min • August 31st, 2013
August 31st, 2013 •
1, 1, 2, 3, 4, 3,5
1Greenebaum Cancer Center, University of Maryland School of Medicine, 2Program in Molecular Medicine, University of Maryland School of Medicine, 3Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine, 4Department of Pharmacology & Experimental Therapeutics, University of Maryland School of Medicine, 5Department of Pathology and Biochemistry & Molecular Biology, University of Maryland School of Medicine

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We ontwikkelden een kwantitatieve DNA-bindende ELISA gebaseerde bepaling om transcriptiefactor interactie met DNA te meten. Hoge specificiteit voor de RUNX2 eiwit werd bereikt met een consensus DNA-herkenning oligonucleotide en specifieke monoklonale antilichaam. Colorimetrische detectie met een enzym-gekoppelde antilichaam substraat reactie werd gevolgd in real time.

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Cellular Biology transcriptiefactoren vitamine D Drug Discovery Enzymgekoppelde Immunosorbent Assay (ELISA) DNA-bindende transcriptiefactor drug discovery antilichaam
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