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Chapters
- 00:05Title
- 01:34Reductive Methylation of Lysozyme
- 05:09Sample Preparation for NMR Analysis
- 08:01Aminopeptidase Degradation of Lysozyme
- 09:34Results: NMR and MALDI MS Analysis of Reductively Methylated Lysozyme Samples
- 13:38Conclusion
Two methods for assigning the α- and ε-dimethylamine nuclear magnetic resonance signals of a reductively 13C-methylated N-terminal lysine are described. One method utilizes the pH-induced selectivity of the reductive methylation reaction, and the other uses aminopeptidase to selectively remove the N-terminal lysine.
