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Immunology and Infection

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Isolering av myeloida dendritiska celler och epitelceller från Human Thymus
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Isolering av myeloida dendritiska celler och epitelceller från Human Thymus

Article DOI: 10.3791/50951-v • 09:07 min • September 19th, 2013
September 19th, 2013 •
1,2, 1, 3, 4, 5, 1
1Department of General Neurology, Hertie Institute for Clinical Brain Research, 2Institute of Pharmacology, University of Bern, 3Department of Immunology, University Medical Center Hamburg-Eppendorf, 4Department of Thoracic and Cardiovascular Surgery, University Clinic Tuebingen, 5Department of Neurology, University Hospital Erlangen

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Detta protokoll specificerar en metod för isolering av antigenpresenterande celler från human tymus via olika stegen i enzymatisk spjälkning av vävnaden följt av densitetscentrifugering av enkelcellsuspension och slutligen magnetiskt och / eller FACS-sortering av cellpopulationer av intresse.

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Immunologi Immunsystemet processer biologiska processer immunologi Immunsystemets sjukdomar immunsystem Phenomena Life Sciences (allmän) immunologi mänsklig thymus isolering dendritiska celler Mtec CTEC
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