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Aislamiento de células dendríticas mieloides y células epiteliales de timo humano

Article DOI: 10.3791/50951-v • 09:07 min • September 19th, 2013
September 19th, 2013 •
1,2, 1, 3, 4, 5, 1
1Department of General Neurology, Hertie Institute for Clinical Brain Research, 2Institute of Pharmacology, University of Bern, 3Department of Immunology, University Medical Center Hamburg-Eppendorf, 4Department of Thoracic and Cardiovascular Surgery, University Clinic Tuebingen, 5Department of Neurology, University Hospital Erlangen

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Este protocolo detalla un método para aislar células presentadoras de antígeno de timo humano a través de diferentes etapas de la digestión enzimática del tejido seguido por centrifugación de densidad de la suspensión de células individuales y la clasificación finalmente magnético y / o FACS de las poblaciones de células de interés.

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Inmunología Número 79 Procesos del Sistema Inmunológico Procesos Biológicos inmunología enfermedades del sistema inmunológico Fenómenos del Sistema Inmunológico Ciencias de la Vida (General) la inmunología el timo humano el aislamiento las células dendríticas MTEC CTEC
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