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Chapters
- 00:05Title
- 01:50Recording Set Up
- 02:38Preparation of Unilamellar Vesicles
- 04:10Efflux Measurement
- 05:27Results: Determination of the Unitary Transport Rate of a CLC-type H+/Cl– Exchanger
- 07:11Conclusion
Proteoliposomes are used to study purified channels and transporters reconstituted in a well-defined biochemical environment. An experimental procedure to measure efflux mediated by these proteins is illustrated. The steps to prepare proteoliposomes, perform the recordings, and analyze data to quantitatively determine the functional properties of the reconstituted protein are described.
