Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Lara Stevanato; Caroline Hicks; John D. Sinden

doi:10.3791/52410

Journal

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발생학

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3 차원 문화, 마이크로 RNA의 분석 및 추정 적 표적 유전자에 인간의 신경 줄기 세포 라인의 차별화

JoVE 신문
발생학

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JoVE 신문 발생학

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

3 차원 문화, 마이크로 RNA의 분석 및 추정 적 표적 유전자에 인간의 신경 줄기 세포 라인의 차별화

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9,852 Views

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10:48 min

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April 12, 2015

DOI:

10.3791/52410-v

DOI:

10.3791/52410-v

•

10:48 min

April 12, 2015

•

18 Views

Lara Stevanato¹, Caroline Hicks¹, John D. Sinden¹

¹ReNeuron

Summary

With the intent of characterizing changes in miRNAs on differentiated human neural stem cells (hNSCs) we describe hNSC differentiation on a three dimensional system,the evaluation of changes in microRNA expression by miRNA PCR array, and computational analysis for miRNA target prediction and its validation by dual luciferase assay.