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Journal / Developmental Biology / Visualisatie van Chondrocyte Intercalatie en…
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Developmental Biology

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JoVE Journal Developmental Biology
Visualisatie van Chondrocyte Intercalatie en Directional Proliferatie via Zebrabow Klonale Cell Analysis in het Embryonale Meckel's Kraakbeen
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Visualisatie van Chondrocyte Intercalatie en Directional Proliferatie via Zebrabow Klonale Cell Analysis in het Embryonale Meckel's Kraakbeen

Article DOI: 10.3791/52935-v • 06:40 min • October 21st, 2015
October 21st, 2015 •
*1, *1, 1, 1
1Massachusetts General Hospital, Center for Regenerative Medicine, Harvard Medical School
* These authors contributed equally

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Cel organisatie van craniofaciale botten is al lang de hypothese, maar niet direct zichtbaar. Multispectrale cell labeling en in vivo levende beeldvorming maakt visualisatie van de dynamische cel gedrag in de zebravis onderkaak. Hier hebben we detail het protocol te manipuleren Zebrabow transgene vis en direct waar te nemen cel intercalatie en morfologische veranderingen van chondrocyten in de Meckel's kraakbeen.

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Developmental Biology craniofaciale ontwikkeling craniale neurale confocale microscopie het lot mapping multi-spectrale klonale analyse sox10 Zebrabow zebravis onderkaak Meckel's kraakbeen
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