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一氧化氮和超氧化物歧化酶恩的人脸检测在完整的动脉内皮细胞层
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En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
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一氧化氮和超氧化物歧化酶恩的人脸检测在完整的动脉内皮细胞层

DOI:
10.3791/53718-v

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08:58 min

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February 25, 2016

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, , , ,
1Cardiovascular and Aging Research, Department of Medicine, Division of Physiology, Faculty of Science,University of Fribourg, 2Kidney Control of Homeostasis,Swiss National Centre of Competence in Research, 3System Physiology, Department of Medicine, Division of Physiology, Faculty of Science,University of Fribourg

Chapters

  • 00:05Title
  • 00:52Preparing the Organ Bath System
  • 02:01Isolation of Mouse Aortas
  • 03:03DHE and DAF-2DA Staining
  • 04:47En Face Mounting and Confocal Microscopic Imaging
  • 06:42Results: eNOS-uncoupling in Obese Mice
  • 07:56Conclusion

Summary

자동 번역

자동 번역

在这篇文章中我们将介绍使用荧光染料diaminofluorescein-2双乙酸钠（DAF-2DA）和二氢乙啶（DHE）对恩面对同时检测和细胞内一氧化氮的可视化（NO）和超氧阴离子（O 2的调整相对容易的方法.- ）分别在新鲜分离的肥胖小鼠模型中的完整主动脉。

Tags

Nitric OxideSuperoxideEndothelial LayerIntact ArteriesEn Face DetectionDAF-2DADHECardiovascular PhysiologyEndothelial DysfunctionENOS UncouplingOrgan Bath SystemKrebs-Ringer Bicarbonate BufferAortic RingsDissectionFluorescent Staining

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