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JoVE Journal Genetics
Genom-bred kortlægning af protein-DNA-interaktioner med ChEC-seq in
Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach
Journal (Genetics)
Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach
HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
Journal (Genetics)
HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
 
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Genom-bred kortlægning af protein-DNA-interaktioner med ChEC-seq in

Article DOI: 10.3791/55836-v • 10:43 min • June 3rd, 2017
June 3rd, 2017 •
1, 2
1Basic Sciences Division, Fred Hutchinson Cancer Research Center, 2Department of Biology, Indiana University

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Vi beskriver chromatin endogen spaltning kombineret med høj gennemstrømningssekvensering (ChEC-seq), en chromatinimmunpræcipitation (ChIP) -orthogonal metode til kortlægning af proteinbindingssteder, som er genomsamlede med mikrokocelle nuklease (MNase) fusionsproteiner.

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Genetik udgave 124 ChEC-seq ChIP-seq transkription genom-bred mediator,
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