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Generation af undslippe varianter af neutraliserende Influenza Virus monoklonale antistoffer
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies

Generation af undslippe varianter af neutraliserende Influenza Virus monoklonale antistoffer

DOI:
10.3791/56067-v

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07:55 min

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August 29, 2017

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, , , , , , ,
1Department of Microbiology,Icahn School of Medicine at Mount Sinai, 2Graduate School of Biomedical Sciences,Icahn School of Medicine at Mount Sinai, 3The Department of Medicine, Section of Rheumatology, The Knapp Center for Lupus and Immunology Research,The University of Chicago

Chapters

  • 00:05Title
  • 00:49Protocol 1: Generation of Escape Mutant Variants Against HI-positive/Neutralization-positive Antibodies
  • 02:51Protocol 2: Generation of Escape Mutant Variants Against HI-negative/Neutralization-positive Antibodies
  • 06:17Results: Epitope Map of the Novel Avian H7N9 HA Generated with Escape Mutant Variants
  • 07:11Conclusion

Summary

자동 번역

자동 번역

Vi beskriver en metode, hvormed vi identificere kritiske restkoncentrationer kræves for bindingen af menneskelige eller murine monoklonale antistoffer, der er målrettet den virale hemagglutinin af influenza A virus. Protokollen kan tilpasses til andre virus overflade glykoproteiner og deres tilsvarende neutraliserende antistoffer.

Tags

Neutralizing AntibodiesMonoclonal AntibodiesEpitope MappingVirus Escape VariantsHemagglutination Inhibition (HI) AssayVirus NeutralizationEmbryonated Chicken EggsMDCK CellsVirus PassagingVirus-antibody Interactions

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