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Journal / Developmental Biology / Bruk av en rekombinant Mosquito Densovirus som et …
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Developmental Biology

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JoVE Journal Developmental Biology
Bruk av en rekombinant Mosquito Densovirus som et gen levering vektor for funksjonell analyse av gener i Larvene
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Bruk av en rekombinant Mosquito Densovirus som et gen levering vektor for funksjonell analyse av gener i Larvene

Article DOI: 10.3791/56121-v • 12:30 min • October 6th, 2017
October 6th, 2017 •
1, 1, 2, 1, 1
1Department of Pathogen Biology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, 2Reproductive Medical Centre of Guangdong Women and Children's Hospital

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Vi rapporterer bruker en kunstig intronic liten RNA uttrykk strategi for å utvikle en ikke-defekt rekombinant Aedes aegypti densovirus (AaeDV) i vivo leveringssystem. En detaljert prosedyre for konstruksjon, emballasje og kvantitativ analyse av rAaeDV vektorer så vel som for larver infeksjon er beskrevet.

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Utviklingspsykologi biologi problemet 128 mygg densovirus gen levering vektor liten RNA, Aedes albopictus vektor kontroll kunstige intron.
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