샘플 준비 및 제 브라에서 RNASeq 기반 유전자 표현 데이터의 분석
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11:42 min
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October 27, 2017
DOI:
Summary
Automatically generated
이 프로토콜에서 zebrafish 태아, 애벌레, 전체 transcriptome 분석에 대 한 접근을 제공 하거나 셀을 정렬 합니다. 우리는 RNA의 격리, RNASeq 데이터의 통로 분석 및 유전자 표정 변화의 qRT-PCR 기반 유효성 검사를 포함합니다.
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Cite this Article
Hostelley, T. L., Nesmith, J. E., Zaghloul, N. A. Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish. J. Vis. Exp. (128), e56187, doi:10.3791/56187 (2017).
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