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Biochemistry

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Quantification de l’abondance et la recharge des niveaux d’ARN de transfert chez Escherichia coli
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Quantification de l’abondance et la recharge des niveaux d’ARN de transfert chez Escherichia coli

Article DOI: 10.3791/56212-v • 10:34 min • August 22nd, 2017
August 22nd, 2017 •
1, 1, 1
1Department of Biology, University of Copenhagen

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Nous présentons ici une méthode pour mesurer directement les ARN de transfert, chargement des niveaux purifiée Escherichia coli RNA, mais aussi un moyen de comparer les niveaux relatifs des ARN de transfert, ou tout autre ARN court, entre les différents échantillons basées sur l’ajout de l’épi cellules exprimant un gène de référence.

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Biochimie numéro 126 Escherichia coli acides ribonucléiques non codantes RNA RNA purification ARN de transfert par Northern blot quantification de RNA aminoacylation niveau de charge.
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