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Chapters
- 00:04Title
- 00:33Incorporating i5 and i7 Adapters onto Error-corrected Sequencing Libraries
- 03:36Quantifying the Libraries with QX200 Digital Droplet PCR (ddPCR)
- 06:14Amplifying and Normalizing the Libraries for Sequencing
- 07:37Results: Mutation-calling with Error-corrected Sequencing (ECS)
- 09:14Conclusion
Next-generation sequencing (NGS) is a powerful tool for genomic characterization that is limited by the high error rate of the platform (~0.5–2.0%). We describe our methods of error-corrected sequencing that allow us to obviate the NGS error rate and detect mutations at variant allele fractions as rare as 0.0001.
