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Immunology and Infection

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JoVE Journal Immunology and Infection
Encellede kvantitering af mRNA og overflade Protein udtryk i Simian immundefektvirus-inficerede CD4+ T celler isoleret fra Rhesus makakaber
The Utilization of Oropharyngeal Intratracheal PAMP Administration and Bronchoalveolar Lavage to Evaluate the Host Immune Response in Mice
Journal (Immunology and Infection)
The Utilization of Oropharyngeal Intratracheal PAMP Administration and Bronchoalveolar Lavage to Evaluate the Host Immune Response in Mice
Particle Agglutination Method for Poliovirus Identification
Journal (Immunology and Infection)
Particle Agglutination Method for Poliovirus Identification
 
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Encellede kvantitering af mRNA og overflade Protein udtryk i Simian immundefektvirus-inficerede CD4+ T celler isoleret fra Rhesus makakaber

Article DOI: 10.3791/57776-v • 13:13 min • September 25th, 2018
September 25th, 2018 •
1, 1, 1, 2, 1
1US Military HIV Research Program, Henry M. Jackson Foundation, Walter Reed Army Institute of Research, 2Vaccine Research Center, NIAID, NIH

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Beskrevet er en metode til at kvantificere udtryk for 96 gener og 18 overflade proteiner af enkelt celler ex vivo, giver mulighed for identifikation af varierende udtrykte gener og proteiner i virus-inficerede celler i forhold til ikke-inficerede celler. Vi anvender tilgang til undersøgelse SIV-inficerede CD4+ T celler isoleret fra rhesus makakaber.

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Immunologi og infektion sag 139 enkelt celle SIV mRNA multipleksede qPCR indekseret flowcytometri rhesus makakaber
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