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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Encellede kvantifisering av mRNA og overflaten Protein uttrykk i Simian immunsvikt-Virus-infiserte CD4⁺ T celler isolert fra Rhesus aper

Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes

Journal (Immunology and Infection)

Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Journal (Immunology and Infection)

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

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Encellede kvantifisering av mRNA og overflaten Protein uttrykk i Simian immunsvikt-Virus-infiserte CD4⁺ T celler isolert fra Rhesus aper

Article DOI: 10.3791/57776-v • 13:13 min • September 25th, 2018

September 25th, 2018 •

Andrey Tokarev¹, Matthew Creegan¹, Michael A. Eller¹, Mario Roederer², Diane L. Bolton¹

¹US Military HIV Research Program, Henry M. Jackson Foundation, Walter Reed Army Institute of Research, ²Vaccine Research Center, NIAID, NIH

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Beskrevet er en metode for å quantitate uttrykket av 96 gener 18 overflaten proteiner av enkeltceller ex vivo, tillater for identifikasjon av ulikt uttrykt gener og proteiner i virus-infiserte celler i forhold til uninfected celler. Vi bruker tilnærming til studiet SIV-infiserte CD4⁺ T celler isolert fra rhesus aper.

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Immunologi og infeksjon problemet 139 enkeltcelle SIV mRNA multiplekset qPCR indekserte flowcytometri rhesus aper

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