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Journal / Immunology and Infection / Encelliga kvantitering av mRNA och ytan…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Encelliga kvantitering av mRNA och ytan proteinuttryck i Simian Immunodeficiency Virus-infekterade CD4+ T celler isolerade från Rhesus makaker
Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 4. Medical Imaging Procedures
Journal (Immunology and Infection)
Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 4. Medical Imaging Procedures
Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
Journal (Immunology and Infection)
Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
 
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Encelliga kvantitering av mRNA och ytan proteinuttryck i Simian Immunodeficiency Virus-infekterade CD4+ T celler isolerade från Rhesus makaker

Article DOI: 10.3791/57776-v • 13:13 min • September 25th, 2018
September 25th, 2018 •
1, 1, 1, 2, 1
1US Military HIV Research Program, Henry M. Jackson Foundation, Walter Reed Army Institute of Research, 2Vaccine Research Center, NIAID, NIH

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Beskrivs är en metod för att kvantifiera uttrycket av 96 gener och 18 ytproteiner genom singelcellerna ex vivo, möjliggör identifiering av differentially uttryckt gener och proteiner i virus-infekterade celler i förhållande till icke-infekterade celler. Vi tillämpar en strategi för studien SIV-infekterade CD4+ T celler isolerade från rhesus makaker.

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Immunologi och infektion fråga 139 enstaka cell SIV mRNA multiplexed qPCR indexerade flödescytometri rhesus makaker
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