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Isolering av Mouse epidermal keratinocytter og deres in vitro Clonogenic kultur

Article DOI: 10.3791/58701-v • 06:16 min • August 10th, 2019
August 10th, 2019 •
1, 1, 1, 1, 1, 1, 1, 1, 1
1Hormel Institute, University of Minnesota

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Målet med denne protokollen er å isolere epidermal keratinocytter fra rygg huden til voksne mus for en rekke nedstrøms anvendelser som molekylærbiologi, biokjemi, fluorescens aktivert celle sortering og primær in vitro-bruk (f.eks. clonogenic keratinocytter).

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Biologi epidermal keratinocytter cellekultur voksen mus epidermis klonal kultur Keratinocyte stamceller 3T3 Fibroblast
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