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Isolering af mus epidermal keratinocytter og deres in vitro-Clonogen kultur
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Isolering af mus epidermal keratinocytter og deres in vitro-Clonogen kultur

Article DOI: 10.3791/58701-v • 06:16 min • August 10th, 2019
August 10th, 2019 •
1, 1, 1, 1, 1, 1, 1, 1, 1
1Hormel Institute, University of Minnesota

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Formålet med denne protokol er at isolere epidermal keratinocytter fra rygskind fra voksne mus til en række downstream-applikationer såsom molekylær biologi, biokemi, fluorescens aktiveret celle sortering og primær in vitro-brug (f. eks. clonogen keratinocytter).

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Biologi epidermal keratinocytter cellekultur voksne mus epidermis klonal kultur keratinocyt stamceller 3T3 fibroblast
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