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Journal / Immunology and Infection / Identificación de ratón y anticuerpos humanos…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Identificación de ratón y anticuerpos humanos repertorios por secuenciación de próxima generación
Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies
Journal (Immunology and Infection)
Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies
Generation and Identification of GM-CSF Derived Alveolar-like Macrophages and Dendritic Cells From Mouse Bone Marrow
Journal (Immunology and Infection)
Generation and Identification of GM-CSF Derived Alveolar-like Macrophages and Dendritic Cells From Mouse Bone Marrow
 
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Identificación de ratón y anticuerpos humanos repertorios por secuenciación de próxima generación

Article DOI: 10.3791/58804-v • 08:51 min • March 15th, 2019
March 15th, 2019 •
1, 2, 3, 1, 4,5, 4, 4, 6, 7,8, 4,5, 9, 2, 9,10
1Graduate School of Life and Environmental Sciences, University of Tsukuba, 2Center for Influenza Virus Research, National Institute of Infectious Diseases, 3School of Science and Technology, Kwansei Gakuin University, 4Department of Pathology, National Institute of Infectious Diseases, 5Division of Infectious Diseases Pathology, Department of Global Infectious Diseases, Tohoku University Graduate School of Medicine, 6Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, 7Division of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, 8Global Station for Zoonosis Control, GI-CoRE, Hokkaido University, 9Department of Immunology, National Institute of Infectious Diseases, 10Faculty of Life and Environmental Sciences, University of Tsukuba

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Aquí, describimos protocolos para el análisis y visualización de la estructura y Constitución de los repertorios de anticuerpos todo. Se trata de la adquisición de grandes secuencias de RNA anticuerpos mediante secuenciación de próxima generación.

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Inmunología e infección número 145 repertorio de anticuerpos secuenciación de próxima generación NGS RNA-seq respuesta inmune reconocimiento del antígeno V-D-J recombinación inmunoglobulina
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