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Identification des substrats de Kinase CK2 roman en utilisant une approche biochimique polyvalente

Article DOI: 10.3791/59037-v • 11:11 min • February 21st, 2019
February 21st, 2019 •
1, 1, 1
1Department of Biochemistry and Molecular Biology, Drexel University College of Medicine

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L’objectif de ce protocole est à étiqueter, enrichir et identifier les substrats de la protéine-kinase CK2 auprès d’un échantillon biologique complex tel qu’un lysat cellulaire ou l’homogénat tissulaire. Cette méthode s’appuie sur des aspects uniques de la CK2 biologie à cet effet.

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Biochimie numéro 144 caséine kinase 2 CK2 protéine kinase identification de substrat phosphorylation analyse de la kinase biologie chimique
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