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Biochemistry

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JoVE Journal Biochemistry
Identificación de los sustratos de quinasa CK2 novela utilizando un enfoque bioquímico versátil
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Identificación de los sustratos de quinasa CK2 novela utilizando un enfoque bioquímico versátil

Article DOI: 10.3791/59037-v • 11:11 min • February 21st, 2019
February 21st, 2019 •
1, 1, 1
1Department of Biochemistry and Molecular Biology, Drexel University College of Medicine

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El objetivo de este protocolo es la etiqueta, enriquecer e identificar los sustratos de la proteína quinasa CK2 de una muestra biológica compleja como un lisado de célula o tejido homogeneizado. Este método aprovecha los aspectos únicos de la biología de CK2 para este propósito.

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Bioquímica número 144 caseína quinasa 2 CK2 quinasa identificación del sustrato fosforilación análisis de la cinasa Biología química
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