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Pretargeted Radioimmunotherapy basato sulla reazione di Diels-Alder domanda inversa Electron

Article DOI: 10.3791/59041-v • 09:44 min • January 29th, 2019
January 29th, 2019 •
1,2, 1,2,3, 1,2,3,4
1Department of Chemistry, Hunter College of the City University of New York, 2Ph.D. Program in Chemistry, Graduate Center of the City University of New York, 3Department of Radiology, Memorial Sloan Kettering Cancer Center, 4Department of Radiology, Weill Cornell Medical College

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Questo protocollo descrive la sintesi e la caratterizzazione di un trans- ciclottene (TCO)-anticorpo e un radioligand tetrazine Lu-etichettati (Tz) 177per pretargeted radioimmunotherapy (PRIT) modifica. Inoltre, vi si descrive l'uso di questi due costrutti di biodistribuzione in vivo e studi longitudinali terapia in un modello murino di cancro colorettale.

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Chimica numero 143 radioimmunotherapy radioimmunotherapy pretargeted pretargeting reazione di Diels-Alder domanda inversa elettroni tetrazine trans- ciclottene huA33 antigene A33 lutetium-177
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