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Journal / Genetics / ATAC-Seq-Assay mit niedrigen mitochondriale DNA…
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Genetics

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ATAC-Seq-Assay mit niedrigen mitochondriale DNA Kontamination von primären menschlichen CD4 + T-Lymphozyten
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ATAC-Seq-Assay mit niedrigen mitochondriale DNA Kontamination von primären menschlichen CD4 + T-Lymphozyten

Article DOI: 10.3791/59120-v • 08:36 min • March 22nd, 2019
March 22nd, 2019 •
1, 1,2, 1,3
1Department of Biology, Boston University, 2Department of Computer Science and Engineering, University of California San Diego, 3Bioinformatics Program, Boston University

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Hier präsentieren wir ein Protokoll, um eine Probe für Transposase zugänglichen Chromatin Sequenzierung (ATAC-Seq) auf aktivierten CD4 + menschlichen Lymphozyten durchführen. Das Protokoll wurde modifiziert, um Kontamination mitochondriale DNA liest von 50 % auf 3 % durch die Einführung eines neuen Lyse-Puffers zu minimieren.

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Genetik Ausgabe 145 Epigenomics ATAC-Seq Tn5 CD4 +-Lymphozyten Kerne mitochondriale DNA-Kontaminationen
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