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Murine Precision-Cut Liver Slices como um modelo ex vivo de biologia hepática

Article DOI: 10.3791/60992-v • 12:36 min • March 14th, 2020
March 14th, 2020 •
*1, *1, 2,3, 1,4,5, 6, 7, 8,9, 2,10, 1,4
1Hepatic Fibrosis Group, QIMR Berghofer Medical Research Institute, 2School of Pharmacy and Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, 3School of Veterinary and Life Sciences, Murdoch University, 4School of Medicine, The University of Queensland, 5Madrid Institute for Advanced Studies (IMDEA) in Food, CEI UAM+CSIC, 6School of Medicine, Griffith University, 7School of Biological Sciences, Queen's University Belfast, 8Department of Gastroenterology & Hepatology, Fiona Stanley and Fremantle Hospital Group, 9School of Medical and Health Sciences, Edith Cowan University, 10Centre for Cell Therapy and Regenerative Medicine, School of Biomedical Sciences, University of Western Australia
* These authors contributed equally

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Este protocolo fornece um método simples e confiável para a produção de fatias hepáticas de corte de precisão viável de camundongos. As amostras de tecido ex vivo podem ser mantidas condições de cultura de tecidos de laboratório por vários dias, fornecendo um modelo flexível para examinar a patobiologia hepática.

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Medicina Edição 157 fígado fatias vibratome ex vivo hepatócitos tecido cultura células estelares
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