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Murine Precision-Cut leverskivor som en Ex Vivo modell av leverbiologi

Article DOI: 10.3791/60992-v • 12:36 min • March 14th, 2020
March 14th, 2020 •
*1, *1, 2,3, 1,4,5, 6, 7, 8,9, 2,10, 1,4
1Hepatic Fibrosis Group, QIMR Berghofer Medical Research Institute, 2School of Pharmacy and Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, 3School of Veterinary and Life Sciences, Murdoch University, 4School of Medicine, The University of Queensland, 5Madrid Institute for Advanced Studies (IMDEA) in Food, CEI UAM+CSIC, 6School of Medicine, Griffith University, 7School of Biological Sciences, Queen's University Belfast, 8Department of Gastroenterology & Hepatology, Fiona Stanley and Fremantle Hospital Group, 9School of Medical and Health Sciences, Edith Cowan University, 10Centre for Cell Therapy and Regenerative Medicine, School of Biomedical Sciences, University of Western Australia
* These authors contributed equally

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Detta protokoll ger en enkel och tillförlitlig metod för produktion av livskraftiga precisionsskurna leverskivor från möss. Ex vivo vävnadsprover kan upprätthållas under laboratorievävnad kultur villkor för flera dagar, vilket ger en flexibel modell för att undersöka lever patobiologi.

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Medicin lever skivor vibratome ex vivo hepatocyter vävnad kultur stellate celler
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