Queen's University (Belfast)

7 articles published in JoVE

• Medicine

  
  Surgical Porcine Model of Chronic Myocardial Ischemia Treated by Exosome-laden Collagen Patch and Off-pump Coronary Artery Bypass Graft Rishav Aggarwal¹, Annie Shao¹, Koray N. Potel², Laura Hocum Stone¹, Cory Swingen¹, Christin Wright¹, Edward O. McFalls³, Tammy A. Butterick^4,5, Rosemary F. Kelly^{1 1}Department of Surgery, University of Minnesota, ² This study presents a surgical porcine model of chronic myocardial ischemia due to progressive coronary artery stenosis, resulting in impaired cardiac function without infarction. Following ischemia, animals undergo off-pump coronary artery bypass graft with epicardial placement of stem cells-derived exosomes-laden collagen patch. This adjunctive therapy improves myocardial function and recovery.

• Medicine

  
  Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology Michael A. Pearen*¹, Hong Kiat Lim*¹, Francis D. Gratte^2,3, Manuel A. Fernandez-Rojo^1,4,5, Sujeevi K. Nawaratna⁶, Geoffrey N. Gobert⁷, John K. Olynyk^8,9, Janina E. E. Tirnitz-Parker^2,10, Grant A. Ramm^{1,4 1}Hepatic Fibrosis Group, QIMR Berghofer Medical Research Institute, ²School of Pharmacy and Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, ³School of Veterinary and Life Sciences, Murdoch University, ⁴School of Medicine, The University of Queensland, ⁵Madrid Institute for Advanced Studies (IMDEA) in Food, CEI UAM+CSIC, ⁶School of Medicine, Griffith University, ⁷ This protocol provides a simple and reliable method for the production of viable precision-cut liver slices from mice. The ex vivo tissue samples can be maintained under laboratory tissue culture conditions for multiple days, providing a flexible model to examine liver pathobiology.

• Bioengineering

  
  Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes Calvin C. L. Cheung¹, Guanglong Ma¹, Amalia Ruiz¹, Wafa T. Al-Jamal^{1 1} The protocol presents the optimized parameters for preparing thermosensitive liposomes using the staggered herringbone micromixer microfluidics device. This also allows co-encapsulation of doxorubicin and indocyanine green into the liposomes and the photothermal-triggered release of doxorubicin for controlled/triggered drug release.

• Medicine

  
  Standardized Measurement of Nasal Membrane Transepithelial Potential Difference (NPD) George M. Solomon¹, Inez Bronsveld², Kathryn Hayes³, Michael Wilschanski⁴, Paola Melotti⁵, Steven M. Rowe¹, Isabelle Sermet-Gaudelus^{6,7 1}Department of Medicine and the Gregory Fleming James Cystic Fibrosis Center, University of Alabama at Birmingham, ²Department of Pulmonology and Tuberculosis, University Medical Center Utrecht, ³Center for Experimental Medicine, Queens University, Northern Ireland, ⁴Hadassah Hebrew University Medical Center, Jerusalem, ⁵Centro Fibrosi Cistica, Azienda Ospedaliera Universitaria Integrata, ⁶Service de Pneumologie et Allergologie Pédiatriques and Center de Ressources et de Compétence de la Mucoviscidose, Hôpital Necker Enfants Malades, ⁷INSERM U 1151, Institut Necker Enfants Malades Here, we present a standardized protocol to measure the nasal potential difference (NPD). Cystic fibrosis transmembrane conductance regulator (CFTR) and epithelial sodium channel (ENaC) function are evaluated by the change in the voltage across the nasal epithelium after superfusion of solutions that modify ion channel activity, providing an outcome measure.

• Biology

  
  Isolation of Retinal Arterioles for Ex Vivo Cell Physiology Studies Tim M. Curtis*¹, Declan McLaughlin², Michael O'Hare¹, Joanna Kur¹, Peter Barabas¹, Gordon Revolta¹, C. Norman Scholfield³, J. Graham McGeown⁴, Mary K. McGahon*^{2 1} This manuscript describes a straightforward protocol for the isolation of arterioles from the rat retina that can be used in electrophysiological, calcium imaging and pressure myography studies.

• Biochemistry

  
  Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions Myriam Jaraíz-Rodríguez¹, Ana González-Sánchez^1,2, Laura García-Vicente¹, Jose M. Medina¹, Arantxa Tabernero^{1 1}Instituto de Neurociencias de Castilla y León (INCYL), Departamento de Bioquímica y Biología Molecular, Universidad de Salamanca, ² This is a protocol to study intracellular protein-protein interactions based on the biotin-avidin pull-down system with the novelty of combining cell-penetrating sequences. The main advantage is that the target sequence is incubated with living cells instead of cell lysates and therefore the interactions will occur within the cellular context.

• Biology

  
  Preparation of Highly Coupled Rat Heart Mitochondria Irina Gostimskaya¹, Alexander Galkin^{2 1}Faculty of Life Sciences, University of Manchester, ²School of Biological Sciences, Queen's University Belfast We describe а protocol for isolation of pure, highly coupled rat heart mitochondria for functional or structural studies of cellular bioenergetics, biophysical measurements, proteomics or mitochondrial DNA and lipids analysis.