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Análisis de espectrometría de masas para identificar la ubiquitylación de CENP-A con etiqueta EYFP (EYFP-CENP-A)
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Análisis de espectrometría de masas para identificar la ubiquitylación de CENP-A con etiqueta EYFP (EYFP-CENP-A)

Article DOI: 10.3791/61138-v • 09:02 min • June 10th, 2020
June 10th, 2020 •
*1, *2, *3, 1, 1, 1, 2, 3
1MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, 2Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, 3Greehey Children's Cancer Institute, Department of Molecular Medicine, UT Health Science Center San Antonio
* These authors contributed equally

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La ubicuidad CENP-A es un requisito importante para la deposición CENP-A en el centromere, heredada a través de la dimerización entre la división celular, e indispensable para la viabilidad celular. Aquí describimos el análisis de espectrometría de masas para identificar la ubiculación de la proteína CENP-A (EYFP-CENP-A) etiquetada por EYFP.

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Biología Número 160 centromere kinetochore mitosis CENP-A modificación post-traduccional PTM ubiculación análisis de espectrometría de masas
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