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Massaspectrometrieanalyse om alomtegenwoordigheid van EYFP-tagged CENP-A (EYFP-CENP-A) te identificeren

Article DOI: 10.3791/61138-v • 09:02 min • June 10th, 2020
June 10th, 2020 •
*1, *2, *3, 1, 1, 1, 2, 3
1MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, 2Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, 3Greehey Children's Cancer Institute, Department of Molecular Medicine, UT Health Science Center San Antonio
* These authors contributed equally

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CENP-A ubiquitylation is an important requirement for CENP-A deposition at the centromere, inherited through dimerization between cell division, and indispensable to cell viability. Hier beschrijven we massaspectrometrieanalyse om alomtegenwoordigheid van EYFP-tagged CENP-A (EYFP-CENP-A) eiwit te identificeren.

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Biologie centromere kinetochore mitose CENP-A post-translationele modificatie PTM alomtegenwoordigheid massaspectrometrie analyse
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