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Masspektrometri analys för att identifiera Ubiquitylation av EYFP-märkt CENP-A (EYFP-CENP-A)
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Masspektrometri analys för att identifiera Ubiquitylation av EYFP-märkt CENP-A (EYFP-CENP-A)

Article DOI: 10.3791/61138-v • 09:02 min • June 10th, 2020
June 10th, 2020 •
*1, *2, *3, 1, 1, 1, 2, 3
1MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, 2Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, 3Greehey Children's Cancer Institute, Department of Molecular Medicine, UT Health Science Center San Antonio
* These authors contributed equally

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CENP-A ubiquitylation är ett viktigt krav för CENP-A nedfall vid centromeren, ärvt genom dimerization mellan celldelning, och oumbärlig för cellens livskraft. Här beskriver vi massa spektrometri analys för att identifiera ubiquitylation av EYFP-taggade CENP-A (EYFP-CENP-A) protein.

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Biologi Utgåva 160 centromere kinetochore mitomos CENP-A post-translationell modifiering PTM ubiquitylation masspektrometrianalys
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