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Analyse de spectrométrie de masse pour identifier l’ubiquité de cenp-A marqué par EYFP (EYFP-CENP-A)

Article DOI: 10.3791/61138-v • 09:02 min • June 10th, 2020
June 10th, 2020 •
*1, *2, *3, 1, 1, 1, 2, 3
1MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, 2Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, 3Greehey Children's Cancer Institute, Department of Molecular Medicine, UT Health Science Center San Antonio
* These authors contributed equally

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Le CENP-Une ubiquitylation est une exigence importante pour le dépôt de CENP-A au centromère, hérité par dimerisation entre la division cellulaire, et indispensable à la viabilité cellulaire. Ici nous décrivons l’analyse de spectrométrie de masse pour identifier l’ubiquité de la protéine CENP-A (EYFP-CENP-A) marquée par EYFP.

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Biologie Numéro 160 centromere kinétochore mitose CENP-A modification post-translationnelle PTM ubiquitylation analyse de spectrométrie de masse
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