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1Department of Medicine,Indiana University School of Medicine, 2Department of Cellular & Integrative Physiology,Indiana University School of Medicine, 3Division of Nephrology, Department of Medicine,Ohio State University Wexner Medical Center, 4Division of Pathology,Indiana University School of Medicine
Chapters
- 00:05Introduction
- 00:41Cryosectioning and Laser Microdissection (LMD)
- 03:39Results: Representative Human Kidney Regional Transcriptomics Analyses
- 05:05Conclusion
Vi beskriver en protokol for laser mikrodissementering af undersegmenter af den menneskelige nyre, herunder glomerulus, proksimal tubule, tykt stigende lem, opsamlingskanal og interstitium. RNA'et isoleres derefter fra de opnåede rum, og der udføres RNA-sekventering for at bestemme ændringer i transskriptionssignaturen inden for hvert undersegment.
