JoVE Journal
Biology
Biology
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.
챕터
요약
We describe a method to generate saturating transposon mutant libraries in Gram-negative bacteria and subsequent preparation of DNA amplicon libraries for high-throughput sequencing. As an example, we focus on the ESKAPE pathogen, Acinetobacter baumannii, but this protocol is amenable to a wide range of Gram-negative organisms.