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A Multi-Cue Bioreactor to Evaluate the Inflammatory and Regenerative Capacity of Biomaterials under Flow and Stretch

Article DOI: 10.3791/61824-v • 07:51 min • December 10th, 2020
December 10th, 2020 •
1,2, 1,2, 1,2, 1, 1, 1,2, *1,2, *1,2
1Department of Biomedical Engineering, Eindhoven University of Technology, 2Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology
* These authors contributed equally

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요약
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The goal of this protocol is to execute a dynamic co-culture of human macrophages and myofibroblasts in tubular electrospun scaffolds to investigate material-driven tissue regeneration, using a bioreactor which enables the decoupling of shear stress and cyclic stretch.

Tags

Multi-Cue Bioreactor Inflammatory Capacity Regenerative Capacity Biomaterials Flow Stretch Hemodynamics Vascular Tissue Regeneration Mechanical Loads Sheer Stress Cyclic Stretch Tissue Engineered Vascular Grafts Electro Spun Scaffold Silicone Tubing 4-0 Prolene Suture Knot Wire Suture Needle Triangular Shape 30% Ethanol
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