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Journal / Genetics / Direkte Umprogrammierung menschlicher…
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Genetics
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JoVE Journal Genetics
Direkte Umprogrammierung menschlicher Fibroblasten in Myoblasten zur Untersuchung von Therapien für neuromuskuläre Störungen
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Direkte Umprogrammierung menschlicher Fibroblasten in Myoblasten zur Untersuchung von Therapien für neuromuskuläre Störungen

Article DOI: 10.3791/61991-v • 10:28 min • April 3rd, 2021
April 3rd, 2021 •
1, 1, 1, 2, 2,3,4,6, 5, 1,6, 1,6
1Center for Gene Therapy, The Research Institute at Nationwide Children’s Hospital, 2Center for Cardiovascular Research, The Research Institute at Nationwide Children’s Hospital, 3The Heart Center, Nationwide Children’s Hospital, 4Division of Genetic and Genomic Medicine, Nationwide Children’s Hospital, 5Department of Human Genetics, The University of Utah School of Medicine, 6Department of Pediatrics, The Ohio State University

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Dieses Protokoll beschreibt die Umwandlung von Hautfibroblasten in Myoblasten und deren Differenzierung in Myotuben. Die Zelllinien stammen von Patienten mit neuromuskulären Störungen und können zur Untersuchung pathologischer Mechanismen und zur Erprobung therapeutischer Strategien verwendet werden.

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Genetik Ausgabe 170 Reprogrammierung hTERT MyoD Fibroblasten Myoblast neuromuskuläre Störung Therapietests
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