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Chapters
- 00:04Introduction
- 00:55Primary Fixation, Post-Fixation, and Embedding
- 02:46Ultrathin Sectioning of Fibers and Contrasting
- 03:57Imaging
- 04:59Image Analyses
- 07:33Results: Glycogen Content in Fiber
- 07:59Conclusion
A modified post-fixation procedure increases the contrast of glycogen particles in tissue. This paper provides a step-by-step protocol describing how to handle the tissue, conduct the imaging, and use stereological methods to obtain unbiased and quantitative data on fiber type-specific subcellular glycogen distribution in skeletal muscle.
