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Journal / Biochemistry / Cuantificación de la distribución de glucógeno…
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Biochemistry

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Cuantificación de la distribución de glucógeno subcelular en fibras musculares esqueléticas mediante microscopía electrónica de transmisión
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Cuantificación de la distribución de glucógeno subcelular en fibras musculares esqueléticas mediante microscopía electrónica de transmisión

Article DOI: 10.3791/63347-v • 08:32 min • February 7th, 2022
February 7th, 2022 •
1, 2, 3, 3, 2
1Research center for applied health science, University College South Denmark, 2Department of Sports Science and Clinical Biomechanics, University of Southern Denmark, 3Department of Biomedical Sciences, Core Facility for Integrated Microscopy, University of Copenhagen

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Un procedimiento modificado posterior a la fijación aumenta el contraste de las partículas de glucógeno en el tejido. Este documento proporciona un protocolo paso a paso que describe cómo manejar el tejido, realizar las imágenes y utilizar métodos estereológicos para obtener datos imparciales y cuantitativos sobre la distribución de glucógeno subcelular específico del tipo de fibra en el músculo esquelético.

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Bioquímica Número 180
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